Developing a simple, cost-effective proof-of-concept vaccine candidate against EHEC for cattle.

ABSTRACT: Enterohemorrhagic Escherichia coli (EHEC) is a zoonotic pathogen responsible for severe human diseases, including hemorrhagic diarrhea and hemolytic uremic syndrome (HUS), primarily mediated by Shiga toxins (Stx). Intestinal colonization depends on the type III secretion system (T3SS), which induces attaching and effacing (A/E) lesions. Cattle are the main reservoir, and preharvest vaccines are key to reducing human exposure. In this work, we engineered a chimeric antigen EIT (EspA36-192-Intimin653-935-Tir258-361) to be expressed in the periplasm of E. coli BL21, enabling simple extraction by thermal shock. To favor scalable production, the process avoids the use of antibiotics, chemical inducers, and mechanical disruption, while remaining compatible with standard infrastructure and low-co

SUBMITTER: Duarte CM 

PROVIDER: S-EPMC12843572 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature