Sexual Hormones Determination in Biofluids by In-Vial Polycaprolactone Thin-Film Microextraction Coupled with HPLC-MS/MS.

ABSTRACT: The in-vial microextraction technique is emerging as an alternative sample treatment, as it integrates sorbent preparation, adsorption, and desorption of analytes in a single device before instrumental analysis. In this work, the applicability of polycaprolactone polymeric film, recently used for the in-vial microextraction of sex hormones from environmental waters, is studied in a low-capacity format for unconjugated sex hormones determination in biological samples by HPLC-MS/MS. Its performance was evaluated in urine and serum, achieving extraction in a short time (10 and 30 min, in turn) and satisfactory elution with ethanol, with recovery in the range of 65-111% in urine, 55-122% in bovine serum albumin (BSA) solution, and 66-121% in fetal bovine serum (FBS). In the case of protein mat

SUBMITTER: Merlo F 

PROVIDER: S-EPMC12843708 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature