Surfactin-Bacillaene Copathway Engineering Strategy Boosts Fengycin Production and Antifungal Activity in Bacillus velezensis HN-Q-8.

ABSTRACT: Previous studies have demonstrated that Bacillus velezensis HN-Q-8 shows significant inhibitory effects against various plant pathogenic fungi causing potato diseases, primarily attributed to the production of fengycin. However, the low yield of fengycin in wild-type strains limits its practical application, and the influence of its biosynthesis pathway on volatile organic compound production remains unclear. In this study, to enhance fengycin production in Bacillus velezensis HN-Q-8, we applied metabolic engineering by targeting competitive pathways. Specifically, a double mutant (ΔsrfAAΔbaeBE) was constructed by knocking out the surfactin synthase gene srfAA and the bacillaene synthesis gene baeBE. The fengycin yield of the ΔsrfAAΔbaeBE mutant in

SUBMITTER: Gao Y 

PROVIDER: S-EPMC12843716 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature