Detecting and Grouping In-Source Fragments with Low-Energy Stepped HCD, Together with MS³, Increases Identification Confidence in Untargeted LC-Orbitrap Metabolomics of Plantago lanceolata Leaves and P. ovata Husk.

ABSTRACT: Background: Comprehensive and accurate compound composition characterization in natural sources has high relevance in food and nutrition, health and medicine, environmental and agriculture research areas, though profiling of plant metabolites is a challenging task due to the structural complexity of natural products. This study delves into the identification and characterization of compounds within the Plantago genus, leveraging state-of-the-art analytical techniques. Methods: Utilizing an ultra-high-performance liquid chromatography (UHPLC) system in conjunction with Orbitrap™ IQ-X™ Tribrid™ mass spectrometer (MS), we employed a Phenyl-Hexyl HPLC column alongside optimized extraction protocols to analyze both husk and leaf samples. To maximize compound identification, we imp

SUBMITTER: Pedisius V 

PROVIDER: S-EPMC12843812 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature