Comprehensive Analysis of Different Subtypes of Oxylipins to Determine a LC-MS/MS Approach in Clinical Research.

ABSTRACT:

Background/objectives

Different oxylipin subtypes have unique biological properties, requiring effective analytical protocols. However, establishing a complete pathway detection protocol for comprehensive oxylipin analysis is challenging. This study aimed to evaluate the adaptability and specificity of oxylipin subtypes under different extraction schemes and to develop a robust analytical platform for clinical biomarker investigation.

Methods

We revealed the adaptability and specificity of oxylipin subtypes based on different single-step extraction schemes. A high-throughput quantitative automated solid-phase extraction coupled with a liquid chromatography-tandem mass spectrometry (aSPE-LC-MS/MS) analytical platform was established for a broad panel of complex oxylipins. The

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC12843908 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature