Bacillus velezensis RF2 Rescued from Citrus Phyllosphere: Dual Mechanisms and Broad-Spectrum Activity for Controlling Citrus Bacterial Canker.

ABSTRACT: Citrus bacterial canker (CBC), caused by Xanthomonas citri subsp. citri (Xcc), threatens citrus production worldwide. Long-term dependence on copper-based bactericides not only poses environmental risks but also accelerates the emergence of copper-resistant Xcc strains. To develop safe and efficient alternative control strategies, 72 bacterial strains were isolated from the phyllosphere of citrus plants naturally infected by CBC and identified by 16S rRNA sequencing. Using an Xcc-GFP-based screening method, we systematically screened a highly effective strain, which was identified as Bacillus velezensis RF2 (Bv-RF2). Both inhibition zone assays and bioactivity tests of the crude methanolic extract of Bv-RF2 demonstrated stable antibacterial activit

SUBMITTER: Luo RF 

PROVIDER: S-EPMC12843974 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature