Longitudinal, Intra-Individual Stability of Untargeted Plasma and Cerebrospinal Fluid Metabolites.

ABSTRACT:

Background/objectives

Longitudinal metabolomics analysis offers valuable insights into how metabolic pathways change according to age and health status. However, metabolite levels can fluctuate due to biological factors (e.g., age, diet, and health status) and technical factors (e.g., sample handling, storage times, and instrument performance), with some metabolites exhibiting greater sensitivity to these sources of variability than others. This study aimed to characterize the longitudinal and technical stability of untargeted plasma and cerebrospinal fluid (CSF) metabolites and to identify a subset that remains reliable over the extended time scales required for epidemiological research.

Methods

Untargeted ultrahigh-performance liquid chromatography-mass spectrometry (LC-MS

SUBMITTER: Rocha B 

PROVIDER: S-EPMC12844231 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature