Fragmentation Resilience Energy Mass Spectrometry (FREMS): Methods Validation and Compound Differentiation.

ABSTRACT: Fragmentation Resilience Energy Mass Spectrometry (FREMS) builds on the field of energy-resolved mass spectrometry and previously used methods, e.g., Survival Yield. It exploits breakdown energies at near "continuous" ramp (0.2% NCE increments) to offer higher resolution and a reliable method for compound differentiation, contaminant identification and structural elucidation. Implementation of FREMS involves acquiring ion breakdown/formation curves as collision energy is incrementally increased. These curves themselves can be analyzed by several means to give a single metric-Fragmentation Resilience (FR₅₀). This value has been shown to be experimentally interchangeable with the modified-Survival Yield (m-SY₅₀) and the Cross-Intersect (C-I). A full panel of testing on

SUBMITTER: Yevdokimov A 

PROVIDER: S-EPMC12844248 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature