Bactericidal Activity of Selenium Nanoparticles Against a Multidrug-Resistant Pathogen: Mechanistic Hypothesis from Exploratory Proteomics.

ABSTRACT: The antimicrobial resistance crisis necessitates novel therapeutics. Selenium nanoparticles (SeNPs) offer promise, but their precise bactericidal mechanism remains poorly defined. This study aimed to define the antibacterial action of SeNPs synthesized via a green method with ascorbic acid and sodium citrate. The resulting SeNPs were monodisperse (17.8 ± 0.66 nm), crystalline, and highly stable (zeta potential: -69.9 ± 4.3 mV), exhibiting potent bactericidal activity against multidrug-resistant E. coli. To generate a mechanistic hypothesis, we integrated phenotypic analyses with a preliminary, single-replicate proteomic profiling. Recognizing this as an exploratory step, we focused our analysis on proteins with the most substantial changes. This revealed a coherent pattern of a targ

SUBMITTER: Elfeky N 

PROVIDER: S-EPMC12844374 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature