Tropism Profiling of Lentiviral Vector Pseudotypes in Diverse Brain Tumor Models.

ABSTRACT: Background: Lentiviral vectors (LVs) show promise as gene therapy tools for brain tumors, but optimal envelope protein choices for different tumor types have not been determined. Methodology: This study evaluated three pseudotyped LV variants-VSV-GP, FuG-B2, and LCMV-GP-across diverse brain tumor cell lines including glioblastoma (GBM), diffuse intrinsic pontine glioma (DIPG), medulloblastoma, and metastatic brain cancers. Results: VSV-GP and FuG-B2 pseudotypes significantly outperformed LCMV-GP across most tumor types. Both VSV-GP and FuG-B2 demonstrated high transduction efficiency in GBM and DIPG cells, though some cell lines displayed selective preferences for one pseudotype over the other. Medulloblastoma cells were challenging to transduce, with only VSV-GP achie

SUBMITTER: Andersen JK 

PROVIDER: S-EPMC12844675 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature