Development of a Microwell System for Reproducible Formation of Homogeneous Cell Spheroids.

ABSTRACT: Background/Objectives: Three-dimensional (3D) cell cultures are increasingly used because 3D cell aggregates better mimic tissue-level biological mechanisms and support studies of tissue physiology and drug screening. However, existing laboratory methods and commercial microwell platforms often yield inconsistent results and can be error-prone, time-consuming, or costly. The objective of this work was to develop a reproducible, high-yield, and cost-effective approach for generating homogeneous cell aggregates using custom 3D-printed microwell stamps. Methods: Custom conical and semi-spherical microwell stamps were fabricated using 3D printing. Stamp resolution was characterized by scanning electron microscopy (SEM). Negative imprints were cast in polydimethylsiloxane (PDMS),

SUBMITTER: Reina Mahecha MA 

PROVIDER: S-EPMC12844868 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature