Overexpression of BnaMATE43b Improves Resistance to Aluminum Toxicity and Identification of Its Upstream Transcription Factors in Rapeseed (Brassica napus L.).

ABSTRACT: The multidrug and toxic compound extrusion (MATE) protein plays a crucial role in mediating plant responses to aluminum (Al) toxicity. The key candidate gene BnaMATE43b related to Al toxicity stress in rapeseed was identified using GWAS and transcriptome analysis. In this study, the BnaMATE43b gene was cloned and functionally characterized in rapeseed. Compared with wild-type rapeseed (WT), the BnaMATE43b overexpression lines (OE) demonstrated stronger aluminum tolerance, specifically manifested in higher relative elongation of taproots (RETs) and relative total root length (RTRL); under Al toxicity stress, the enzyme activities (SOD and POD) and root activity were significantly increased in the OE lines, whereas the MDA content and relative electrical conductivity wer

SUBMITTER: Xiao X 

PROVIDER: S-EPMC12844873 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature