Peptide MegaPools Approach to Evaluate the Dengue-Specific CD4 and CD8 T-Cell Response.

ABSTRACT:

Background

Being central players in the adaptive immunity, the study of T-cell responses is crucial in both natural infections and vaccine-induced immunity. In this study, we assessed the antigen-specific T-cell responses to dengue virus (DENV) to identify the most immunogenic antigen for evaluating dengue-specific T-cell responses.

Methods

Patients with dengue disease and subjects vaccinated with the QDENGA (TAK-003) vaccine (before and three months after vaccination) were enrolled. The T-cell-specific response was measured by ELISPOT and Activation Induced Markers (AIM) assay following PBMC stimulation either with DENV1-4 CD4 and CD8 MegaPools (MP) or serotype-specific DENV peptide pools at different concentrations.

Results

We found that both DENV1-4 CD4 MP (at 1 µg

SUBMITTER: Tiberi M 

PROVIDER: S-EPMC12845116 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature