Induction and Regeneration of Microspore-Derived Embryos for Doubled Haploid Production in Cabbage (Brassica oleracea var. capitata).

ABSTRACT: Cabbage (Brassica oleracea L. var. capitata) is an important leafy vegetable crop, and the development of homozygous parental lines is essential for F₁ hybrid breeding. Isolated microspore culture (IMC) provides a rapid approach for producing haploid and doubled haploid (DH) lines. However, its efficiency in cabbage remains highly dependent on genotype, donor plant growth conditions, and culture conditions. This study aimed to optimize key factors affecting microspore embryogenesis and plant regeneration in a Korean green cabbage ('SJ-Ca 13') and to evaluate the ploidy and genetic characteristics of regenerated plants. Microspore yield and embryogenesis were strongly influenced by flower bud size. Bud size of 4.0 ± 0.5 mm yielded the highest number of microspores (

SUBMITTER: Choi SB 

PROVIDER: S-EPMC12845209 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature