Developmental Changes in the Expression of Lysophosphatidic Acid Receptor 4 in the Mouse Brain.

ABSTRACT:

Introduction

Lysophosphatidic acid (LPA) is a bioactive phospholipid that mediates a variety of biological actions through binding to G protein-coupled receptors known as LPA receptors (LPARs). In mammals, six LPAR subtypes (LPAR1-6) have been identified. This study aimed to determine the expression of LPAR4 in the developing mouse brain.

Methods

Brains samples were prepared from mice in various stages of development and biochemical and immunohistochemical analyses were conducted using anti-LPAR4.

Results

Western blot analysis detected two LPAR4-immunoreactive species at ∼50 kDa and ∼42 kDa from embryonic day 16.5 (E16.5). The ∼50 kDa molecule increased during development, reaching a peak at postnatal day 3 (P3), and then gradually decreased through P22. In contrast,

SUBMITTER: Ishidou A 

PROVIDER: S-EPMC12845519 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature