RNA N-glycosylation enables immune evasion and homeostatic efferocytosis.

ABSTRACT: Glycosylation is central to the localization and function of biomolecules¹. We recently discovered that small RNAs undergo N-glycosylation² at the modified RNA base 3-(3-amino-3-carboxypropyl) uridine (acp³U)³. However, the functional significance of N-glycosylation of RNAs is unknown. Here we show that the N-glycans on glycoRNAs prevent innate immune sensing of endogenous small RNAs. We found that de-N-glycosylation of cell-culture-derived and circulating human and mouse glycoRNA elicited potent inflammatory responses including the production of type I interferons in a Toll-like receptor 3- and Toll-like receptor 7-dependent manner. Furthermore, we show that N-glycans on cell surface RNAs prevent apoptotic cells from triggering endosomal RNA sen

SUBMITTER: Graziano VR 

PROVIDER: S-EPMC12845878 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature