Compatibility and Stability of a Shigella Polysaccharide-Protein Conjugate Antigen Formulated with Aluminum Salt and CpG 1018^® Adjuvants.

ABSTRACT: This study evaluated the formulation and stability of a quadrivalent glycoconjugate Shigella vaccine candidate based on four predominant strains (S. flexneri; 2a, 3a, and 6, and S. sonnei) covering ~64% of global Shigella infections. Each glycoconjugate antigen consists of a strain-specific O-polysaccharide (O-PS) covalently linked to the carrier protein IpaB, a component of the Shigella type III secretion system. First, selective competitive ELISAs were developed to measure antigenicity of the four O-PS-IpaB conjugates formulated with different adjuvants (i.e., Alhydrogel^®, AH; Adju-phos^®, AP; and CpG-1018^®, CpG). Next, the monovalent S. sonnei O-PS-IpaB conjugate was studied to elucidate inter

SUBMITTER: Taskar P 

PROVIDER: S-EPMC12846156 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature