Efficient Expression of Lactone Hydrolase Cr2zen for Scalable Zearalenone Degradation in Pichia pastoris.

ABSTRACT: Zearalenone (ZEN) is a thermostable, lipophilic, non-steroidal estrogenic mycotoxin produced by Fusarium spp. that persistently contaminates food and feed. Its strong estrogenic activity and resistance to conventional detoxification strategies pose significant threats to food safety and human and animal health. Conventional physical and chemical degradation methods often compromise nutritional quality and leave toxic residues. Here we report the engineering of a novel Clonostachys rosea lactone hydrolase, Cr2zen, for efficient ZEN degradation in Pichia pastoris under mild conditions. Native Cr2zen exhibited a protein concentration of 0.076 mg/mL, achieving a degradation rate of approximately 17.9% within 30 min, with kinetic parameters of K_m 75.9 µM a

SUBMITTER: Ahmad M 

PROVIDER: S-EPMC12846278 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature