Purification, Structural Characterization, and Antibacterial Evaluation of Poly-γ-Glutamic Acid from Bacillus subtilis.

ABSTRACT: Extracellular poly-γ-glutamic acid (γ-PGA) produced by Bacillus species demonstrates significant antibacterial properties, positioning it as a promising candidate for diverse biomedical and industrial applications. This study focused on molecular identification of Bacillus subtilis using Polymerase Chain Reaction (PCR) and evaluated the initial production of γ-PGA from a novel biological source of Bacillus subtilis. Shake flask fermentation was utilized for γ-PGA production, with three distinct growth media (Tryptic, MRS, and Mineral medium) assessed for their efficiency in polymer yield. Characterization of γ-PGA was conducted through FT-IR, HPLC, and GC-MS analyses. FT-IR spectroscopy confirmed the presence of characteristic functional groups such as carbonyl, amide,

SUBMITTER: Chandrakasan G 

PROVIDER: S-EPMC12846282 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature