Transcriptome-Wide Identification and Analysis Reveals m⁶A Regulation of Porcine Intestinal Epithelial Cells Under TGEV Infection.

ABSTRACT: Transmissible gastroenteritis virus (TGEV) represents a critical intestinal pathogen responsible for acute enteritis in pigs, posing significant challenges to global swine production biosecurity. N⁶-methyladenosine (m⁶A), the most abundant epitranscriptomic mark in eukaryotic messenger RNA, has emerged as a regulatory factor in host-virus interactions. Despite its recognized importance, the functional significance of m⁶A modifications during TGEV infection of porcine jejunal epithelial (IPEC-J2) cells remains unexplored. Here, we established a TGEV-infected IPEC-J2 cell model and we employed methylated RNA immunoprecipitation sequencing (MeRIP-seq) to comprehensively profile the m⁶A epitranscriptomic landscape and identify N⁶-methylade

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12846421 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature