Optimized Multi-Epitope Norovirus Vaccines Induce Robust Humoral and Cellular Responses in Mice.

ABSTRACT: Background: Norovirus GII.4 is a major global health threat, yet no licensed vaccines exist due to the virus's rapid evolution and high mutation rates. Objective: To rationally design and experimentally validate multi-epitope vaccine candidates against Norovirus GII.4 using computational immunoinformatics and in vivo evaluation. Methods: We employed reverse vaccinology to screen optimal norovirus GII.4 epitopes and systematically designed four construction strategies to evaluate different epitope topologies and adjuvants. Candidates underwent molecular dynamics simulations and were expressed in E. coli. Immunogenicity was assessed in BALB/c mice via ELISA and ELISPOT to evaluate humoral and cellular responses. Results: Three candidates (NV1, NV4, NV5) were succe

SUBMITTER: Xing Z 

PROVIDER: S-EPMC12846433 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature