Examination of In Vivo Mutations in VP4 (VP8*) of the Rotarix^® Vaccine from Shedding of Children Living in the Amazon Region.

ABSTRACT: Group A rotaviruses (RVAs) remain the leading cause of acute gastroenteritis (AGE) in young children in low- and middle-income countries. In Brazil, the oral attenuated RVA vaccine (Rotarix^®), monovalent genotype G1P[8], is distributed by the national immunization program and has drastically reduced morbidity and mortality associated with RVA etiology. In this study, Rotarix^® G1P[8] was detected using specific qRT-PCR from the fecal shedding of children living in the Amazon region, and 18.3% (29/158) were positive and 75.8% (22/29) presented with AGE. The VP4 (VP8*) gene of these sheddings, submitted to Sanger nucleotide sequencing, showed an occurrence of mutations, including the silent mutation at 144C > G (one child) and the following missense mutations- 499T > C (

SUBMITTER: Silva MF 

PROVIDER: S-EPMC12846572 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature