Parvovirus B19 and Cellular Transcriptome Dynamics in Differentiating Erythroid Progenitor Cells.

ABSTRACT: Parvovirus B19 (B19V) is a human ssDNA virus with ample pathogenic potential. It is characterized by a selective tropism for erythroid progenitor cells (EPC), exerting a cytotoxic effect with blockade of erythropoiesis. In our work, we investigated both viral and cellular expression profile in the course of infection of EPCs cultures via mRNA high throughput sequencing technology (HTS) and a dedicated bioinformatic pipeline, reconstructing both the viral and cellular transcriptome and their variations. A productive infection was confirmed as restricted to EPCs expressing mature differentiation markers and the specific receptor for virus VP1u region. mRNA HTS reconstructed the viral transcriptome in terms of localization and abundance of the different mRNA species, detailing the differentia

SUBMITTER: Fasano E 

PROVIDER: S-EPMC12846583 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature