Coupling proteostasis and de novo purine biosynthesis of PSMD14 fuels glioblastoma progression and chemoresistance.

ABSTRACT: Background: Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor characterized by rapid proliferation, profound invasiveness, and resistance to conventional therapies. Deubiquitinating enzymes (DUBs), crucial regulators of protein homeostasis, have recently been implicated in GBM pathogenesis. However, the specific DUBs that play central roles in GBM pathogenesis and their exact molecular mechanisms remain to be further elucidated. Methods: We systematically analyzed GBM datasets and clinical samples to identify differentially expressed DUBs. Functional experiments, including genetic manipulation, immunoprecipitation coupled mass spectrometry (IP-MS), comprehensive metabolic assays, mitochondrial function assessments, and orthotopic mouse models, were cond

SUBMITTER: Wang J 

PROVIDER: S-EPMC12846754 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature