Exploring the effect of skim milk on the membrane stability of frozen-thawed Inner Mongolia cashmere goat sperm based on proteomics.

ABSTRACT:

Introduction

The cryopreservation of semen from the Inner Mongolia cashmere goat, a valuable dual-purpose breed in China, results in a sharp decline in sperm motility, hindering genetic improvement and germplasm propagation. This study aimed to investigate the protective effects and underlying mechanisms of skim milk as a supplement in a cryopreservation extender.

Methods

Skim milk was added stepwise (2%-3.6%) to an egg yolk-soy lecithin basal extender, with 2.8% identified as the optimal concentration. Tandem mass tag (TMT) quantitative proteomics, coupled with parallel reaction monitoring (PRM) validation, was employed to analyze the proteomic profiles of post-thaw sperm and elucidate homeostatic mechanisms related to sperm membrane stability.

Results

The addition o

SUBMITTER: Xue SH 

PROVIDER: S-EPMC12846951 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature