Spatial transcriptome and single-cell sequencing reveal the role of nucleotide metabolism in breast cancer progression and tumor microenvironment.

ABSTRACT:

Background

The complexities of nucleotide metabolism in breast cancer (BC) cells are not yet fully understood. A deeper exploration of the various tumor subpopulations and their interactions with the tumor microenvironment (TME) could provide important insights into these clinically relevant signaling pathways.

Methods

We integrated five distinct single-cell enrichment scoring methodologies to conduct a comprehensive enrichment analysis of BC cell populations. The analytical findings underwent subsequent validation using an independent single-cell cohort. Tumor cell clusters were categorized based on their average enrichment scores. Functional analyses were carried out using several tools, including CellChat, Monocle, CopyKAT, SCENIC, and CytoTRACE. The RCTD method was then

SUBMITTER: Pan Y 

PROVIDER: S-EPMC12847018 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature