Enhanced initiation of somatic embryos in suspension cultures of Aesculus flava and metabolic profile of zygotic embryos and somatic embryos during their development.

ABSTRACT: Zygotic embryos (ZEs) of Aesculus species contain a plethora of health-promoting phytochemicals that are used in the pharmaceutical industry and traditional medicine. However, the seed yield decreases due to various stresses, and seeds often contain elevated levels of heavy metals as they are mostly collected from urban environments. Somatic embryos (SEs) could serve as an alternative source of these phytochemicals. Therefore, this study aimed to develop protocols for efficient initiation, regeneration, proliferation, and cryopreservation of A. flava SEs, while also establishing a metabolic profile of ZEs and SEs at successive developmental stages. The frequency of initiation of SEs from the filament-derived friable callus was approximately eightfold higher for explants cultu

SUBMITTER: Zdravkovic-Korac S 

PROVIDER: S-EPMC12847295 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature