The differential expression profiles of miRNA in serum-derived exosomes and its potential role in age-related hearing loss.

ABSTRACT:

Objectives

This study aimed to characterize serum exosomal miRNA profiles from patients with age-related hearing loss (ARHL) to identify key pathogenesis-related miRNAs for ARHL.

Methods

Peripheral venous blood samples were collected from patients with ARHL and elderly controls, and exosomes were isolated from serum of each subject. Then, the isolated exosomes were systematically identified by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and western blot. Subsequently, the isolated exosomes were submitted for miRNA sequencing and a series of bioinformatics analysis. Ultimately, four key DE-miRNAs, namely hsa-miR-100-5p, hsa-miR-23b-3p, hsa-miR-373-3p, and hsa-miR-27b-3p, were verified using quantitative real-time polymerase chain reaction (RT-

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12847408 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature