An integrated subtractive genomics and immunoinformatic approach for designing a multi-epitope peptide vaccine against methicillin-resistant Staphylococcus aureus.

ABSTRACT:

Introduction

MRSA is a multi-drug-resistant bacteria responsible for severe infections that has become a major health concern. Due to constraints of traditional methods, there is a need for developing a new approach to prevent the MRSA-related infections by targeting key pathogens.

Methods

Initially, the subtractive genomics was applied to the MRSA proteome to identify non-homologous, essential, and virulence targets using comparative BLAST-based screening. Further, immunoinformatic tools were employed for B- and T-cell epitope prediction and vaccine construction with appropriate adjuvants and linkers, followed by immune simulation and molecular docking with immune receptors.

Results

Comparative metabolic pathway analysis identified 294 MRSA pathway proteins, with ace

SUBMITTER: Subramani NK 

PROVIDER: S-EPMC12847441 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature