CTHRC1/TGF-β/Smad axis serves as therapeutic targets in inhibiting oral squamous cell carcinoma by mediating ferroptosis.

ABSTRACT:

Background

Collagen triple helix repeat containing 1 (CTHRC1) is an oncogene in numerous human cancers including oral squamous cell carcinoma (OSCC). However, whether and how CTHRC1 influences OSCC progression through ferroptosis has not been completely clarified.

Methods

qRT-PCR were conducted to assess CTHRC1 expression in OSCC. The proliferation of OSCC cells were assessed through 5-ethynyl-2'-deoxyuridine (EdU) assay, colony formation, and CCK-8 assays. Flow cytometry was used to analyze the apoptotic cells of OSCC cells. Ferroptosis was assessed based on the amounts of MDA, lipid ROS, intracellular Fe²⁺, and ferroptosis-related proteins. TGF-β/Smad pathway-related protein were detected by Western blot.

Results

Pronounced overexpression of CTHRC1 was ob

SUBMITTER: Huang S 

PROVIDER: S-EPMC12847467 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature