AsAP2 transcriptionally activates ferulate 5-hydroxylase, diverting ferulic acid metabolism toward lignin biosynthesis in Angelica sinensis.

ABSTRACT: Premature flowering in Angelica sinensis (Danggui) triggers severe declines in root yield and medicinal quality5 by reducing bioactive ferulic acid and promoting lignification. Ferulate 5-hydroxylase (F5H), a cytochrome P450 enzyme, drives metabolic flux toward lignin biosynthesis, but its regulatory network and functional dynamics during early flowering remain unresolved. Transcriptomic and functional analyses identified AsF5H (As09G05225) as a key gene upregulated in early-flowering plants, correlating with root lignin accumulation and ferulic acid depletion. Phylogenetic studies confirmed F5H functional conservation across Apiaceae species, while heterologous expression in yeast validated its enzymatic activity in converting ferulic acid to 5-hydroxyferulic acid. Structural modeling pin

SUBMITTER: Wu Z 

PROVIDER: S-EPMC12847691 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature