Integration of human ERKs and DUSPs into the yeast cell wall integrity pathway.

ABSTRACT: The Saccharomyces cerevisiae Cell Wall Integrity (CWI) pathway responds to cell wall stress and is composed of MAP3K Bck1, MAP2Ks Mkk1 and Mkk2 and MAPK Slt2. Although human ERK5 has been considered the functional orthologue of Slt2, our results indicate that human ERK1 and ERK2 exhibit a much greater ability than ERK5 to replace Slt2 under various cell wall stresses. ERK5 is only able to slightly complement an slt2Δ mutant phenotype in the presence of tunicamycin, and the constitutively active truncated version ERK5ΔCt did not improve this complementation ability. Like Slt2, ERK1, ERK2 and ERK5ΔCt are concentrated in the nucleus, and show higher phosphorylation than ERK5 upon CWI pathway stimulation. Expression of a hyperactive version of the human MAP2K MEK5 leads to specific ERK5 and ER

SUBMITTER: Lavilla-Garcia B 

PROVIDER: S-EPMC12847733 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature