Chicken biliary exosomes ameliorate lipopolysaccharide-induced hepatocyte injury.

ABSTRACT: Investigate the protective effects of chicken bile-derived exosomes (CB-exos) against lipopolysaccharide (LPS)-induced acute hepatocyte injury. CB-exos were characterized by transmission electron microscopy observation and Western blotting. Subsequently, after 24 h of CB-exos treatment, the levels of alanine transaminase (ALT) and aspartate transaminase (AST) in the hepatocyte culture supernatant and chicken serum were measured using specific test kits. The morphology of hepatocytes was observed under a microscope. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to detect the expression levels of inflammatory cytokines, apoptosis-related proteins, and hepatocyte growth factors. CB-exos exhibited a typical cup-shaped morphology with an average diameter

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC12847773 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature