Tubule-derived CCN1 drives renal repair via α_vβ₅-STAT6-ARG1-dependent reprogramming of macrophages.

ABSTRACT: Macrophages play a critical role in injury and repair following acute kidney injury (AKI), but their regulatory mechanisms remain incompletely understood. Cellular communication network factor 1 (CCN1), a secreted matricellular protein and early biomarker of AKI, may regulate macrophage function during kidney injury. In this study, we first investigated CCN1's interaction with macrophages in a murine model of ischemia-reperfusion (I/R)-induced AKI. The role of CCN1 was further investigated using recombinant protein administration, a renal tubular epithelial cell (RTEC)-specific CCN1 knockdown mouse model via adeno-associated virus, and in vitro studies with bone marrow-derived macrophages (BMDMs). We found that in response to injury, RTECs upregulated and secreted CCN1, which colocalized w

SUBMITTER: Zhang N 

PROVIDER: S-EPMC12847786 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature