DNA repair pathway-related proteins are involved in the circularization step of microDNA eccDNA^fib-L.

ABSTRACT: A large number of extrachromosomal circular DNAs (eccDNAs) are found in eukaryotic cells, but the mechanism behind the formation of eccDNAs remains unknown. EccDNAs smaller than 1000 bp are commonly referred to as microDNAs. In a prior study, we identified a 542 bp microDNA, designated eccDNA^fib-L, mapping to Chromosome 14: 9,692,083-9,692,624 nt in the silk gland of Bombyx mori. There is a direct short repeat "GAGT" at both 5' and 3' break points of eccDNA^fib-L, but only one copy of "GAGT" is retained in eccDNA^fib-L. Here, we find that the specific junction observed can also be detected by transfecting with a DNA fragment containing upstream sequences of the 5' break point and downstream sequences of the 3' break point of eccDNA^fib-L. Additional

SUBMITTER: Tong X 

PROVIDER: S-EPMC12847890 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature