LLPS droplet size estimation via UV-Vis spectroscopy using a microplate reader.

ABSTRACT: Liquid-liquid phase separation (LLPS) is critical to the formation of membraneless organelles within cells. Monitoring the size and dynamics of LLPS droplets is essential to understanding their function. However, conventional methods, such as microscopy and dynamic light scattering (DLS), have limitations, including low throughput and complex setups. Here, we present a simple, label-free, high-throughput method for estimating droplet size using ultraviolet-visible (UV-Vis) spectra obtained using a standard microplate reader. This method is theoretically supported by Mie scattering theory. By analyzing the spectral shape and comparing it to that of glass beads of known sizes, our method enables the quantitative estimation of LLPS droplet size in a minimally invasive manner. We validated our

SUBMITTER: Enomoto-Kusano M 

PROVIDER: S-EPMC12847920 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature