Chemical and photoinduced interstrand crosslinking of oligo DNA duplexes containing 2'-deoxythioguanosines.

ABSTRACT: Interstrand crosslinked nucleic acids offer diverse applications, including stabilizing DNA nanostructures in nanotechnology, enhancing miRNA inhibition in therapeutics, and studying enzyme activity in biochemistry. In this study, we present a new crosslinking design strategy that incorporates two 2'-deoxythioguanosine residues at noncomplementary yet strategically proximal positions within an oligo DNA duplex, promoting efficient disulfide crosslinking under chemical oxidation conditions. Additionally, this approach enables a photoinduced crosslinking reaction using 365 nm UV light, further facilitating the formation of disulfide crosslinked duplex. Our results demonstrate that the proximity of reactive thiocarbonyl groups of 2'-deoxythioguanosines incorporated within the duplex favors di

SUBMITTER: Osman JA 

PROVIDER: S-EPMC12847958 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature