Rhomboid protease GlpG regulates type 1 pili quality control and virulence in pathogenic E. coli.

ABSTRACT: With the rise in antimicrobial resistance, understanding the virulence factors utilized by pathogenic E. coli is essential for the development of alternative therapeutics. While previous work has shown that disruption of the E. coli rhomboid protease gene glpG leads to defects in bacterial colonization, here we provide mechanistic insight into the loss of fitness. We show GlpG is essential for the assembly of type 1 pili, a virulence factor required for the colonization of eukaryotic cells. Since pili are critical for biofilm formation and bacterial persistence, the absence of GlpG proteolytic activity reduces the production of biofilm. Working towards new potential antimicrobial targets for treating infections, we show that biofilm formation is hampered by GlpG inhibition. Our data demons

SUBMITTER: Lu J 

PROVIDER: S-EPMC12847965 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature