¹⁹F NMR study of proteins with parallel incorporation of multiple fluorinated aromatic amino acids.

ABSTRACT: Although the parallel incorporation of fluorinated aromatic amino acids (FAAAs) into proteins has been explored since 2010, the corresponding ¹⁹F NMR investigation has been lacking to date. To assess the potential of the parallel incorporation of FAAAs in studies of protein structure, dynamics and interactions, we examine the protein yields, fluorine incorporation efficiencies, and ¹⁹F NMR spectra upon simultaneously incorporating FAAAs including 3-fluorotyrosine (3FY), 4-fluorophenylalanine (4FF) and 5-fluorotryptophan (5FW) into two model proteins: the structured protein GB1 domain (GB1) and the intrinsically disordered protein α-synuclein (α-syn). We found that the simultaneous incorporation of 3FY and 5FW achieved higher efficiency than combinations of 4FF and 5FW

SUBMITTER: Li M 

PROVIDER: S-EPMC12848272 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature