Rhein Inhibits NLRP3 Activation and Alleviates Microglial Pyroptosis After Intracerebral Hemorrhage in Rats.

ABSTRACT:

Background

Intracerebral hemorrhage (ICH) induces severe neuroinflammation and microglial pyroptosis, exacerbating secondary brain injury. Rhein, a natural anthraquinone compound, possesses anti-inflammatory and neuroprotective properties. However, its effects on microglial pyroptosis and the underlying mechanisms remain unclear.

Methods

A rat microglial (RM) pyroptosis model was established using LPS + ATP induction, followed by rhein intervention and NLRP3 knockdown. Cell proliferation was assessed using CCK-8, and apoptosis was evaluated through TUNEL staining. ELISA was used to measure the expression levels of inflammatory cytokine. Immunofluorescence staining was performed to label M1/M2 microglia. RT-qPCR and western blot were used to analyze the expression of NLRP3, A

SUBMITTER: Aimaiti A 

PROVIDER: S-EPMC12848515 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature