Biocompatible Ink Optimization Enables Functional Volumetric Bioprinting With Xolography.

ABSTRACT: Xolography is a novel linear volumetric manufacturing technique that offers unparalleled precision and speed. Yet, its application to bioprinting remains limited due to insufficient understanding of biocompatibility constraints. Here, this work establishes fundamental design principles for cell-compatible Xolography bioinks by dissecting the effects of extracellular pH, osmolality, and lysosomotropic stress on cell viability and function. By systematically studying the tolerances for these parameters, this work defines a framework for bioink formulations that enables fast, support-free fabrication of complex designs with maintained cell viability and function as validated in different murine and human cell lines, primary human cells and induced pluripotent stem cell (iPSC)-derived cells. T

SUBMITTER: Brauer E 

PROVIDER: S-EPMC12848640 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature