Affinity Maturation of a Fentanyl Aptamer by Motif-SELEX.

ABSTRACT: Oligonucleotide-based aptamers are increasingly being used as recognition elements in biosensors to detect analytes relevant to diverse applications including medical diagnostics and therapeutic drug monitoring. Nevertheless, the utility of aptamers is often hindered by their insufficient target-binding affinity. We recently developed an affinity maturation approach termed Motif-SELEX that can improve aptamer binding affinity by up to an order of magnitude. This approach entails performing in vitro selection with a library containing conserved sequence motifs of an existing aptamer flanked by variable-length random domains that serve to enhance interactions with the target. Here, we demonstrate the application of Motif-SELEX to a DNA aptamer that binds to fentanyl with highalbeit i

SUBMITTER: Alkhamis O 

PROVIDER: S-EPMC12848694 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature