In Vivo Evaluation of Liver Injury and Arthritis Enabled by a Nitroxyl-Activated Near-Infrared Fluorescence and Photoacoustic Dual-Modality Imaging Probe.

ABSTRACT: Nitroxyl (HNO) is the transient form of nitric oxide (NO) and is highly reactive, which makes its in vivo study a significant challenge. We report here simultaneous fluorescence and photoacoustic imaging of HNO, enabled by a newly synthesized near-infrared, dual-modality fluorescence and photoacoustic (FPA) probe. The 2-(diphenylphosphonyl)-benzoate moiety in the FPA probe specifically recognized HNO. The photophysical properties of the probe were tuned, e.g., by substituting an oxygen atom in the hemicyanine moiety with sulfur, resulting in a 45 nm red-shift. The maximum absorption wavelength increased from 650 nm in the absence of HNO to 760 nm in the presence of HNO. We demonstrate an application of the HNO-activated FPA probe to in vivo evaluation of inflammatory disease

SUBMITTER: Liao L 

PROVIDER: S-EPMC12848702 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature