High-throughput single-cell DNA methylation and chromatin accessibility co-profiling with SpliCOOL-seq.

ABSTRACT:

Background

DNA methylation and chromatin accessibility are pivotal epigenetic regulators of gene expression and cellular identity, with significant implications in tumorigenesis and progression. Current single-cell multi-omics methods are limited in throughput and sensitivity, hindering comprehensive biomarker discovery.

Methods

We developed single-cell split-pool ligation-based multi-omics sequencing technology (SpliCOOL-seq), a high-throughput single-cell sequencing technology that simultaneously profiles whole-genome DNA methylation and chromatin accessibility in thousands of cells. By integrating in situ GpC methylation, universal Tn5 tagmentation, and split-pool combinatorial barcoding, SpliCOOL-seq achieves enhanced sensitivity and scalability.

Results

SpliCOOL-

SUBMITTER: Shen Q 

PROVIDER: S-EPMC12848781 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature