Disruption of ATP Synthase Spatiotemporal Organization, Ca²⁺ Dynamics, and Contractile Function in Senescent Cardiomyocytes.

ABSTRACT: Heart disease is the leading cause of death in the elderly population. Age-related heart failure is frequently associated with energy deficits in cardiomyocytes. These cells rely on their abundant, cristae-rich mitochondria for ATP production. ATP synthase, localized along the cristae rims, is central to this process. It is presumed that its function is tightly bound to its spatial organization, but details remain unclear. Here, we explored the spatiotemporal organization of ATP synthase in senescent human iPSC-derived CM in conjunction with its functions. We found changes in the stoichiometry of F₁ and F_O subunits in senescent CM. The ratio of F_O-SU c to F₁-SU β increased. The oligomeric organization of the complex was weakened. Using single-mol

SUBMITTER: Morris S 

PROVIDER: S-EPMC12848789 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature