Silicon Rhodamine-Based Fluorescence Lifetime Probe for Dynamics Mapping Lysosomal Oxidative Stress.

ABSTRACT: Lysosomes are organelles responsible for cellular degradation and recycling. The detection of changes in the lysosomal microenvironment, such as viscosity, oxidative stress, and pH value, as well as their interactions among dynamic organelles, remains an intriguing field that contributes to elucidating intracellular homeostasis. Here, we describe the development of a fluorescent probe tool for uniting fluorescence lifetime imaging microscopy (FLIM) and dual-channel near-infrared (NIR) fluorescence signals, which can simultaneously monitor viscosity and reactive oxygen species (ROS) in lysosomes. SiR-Eda exhibits a viscosity-dependent fluorescence lifetime and ROS-sensitive fluorescence emission, allowing for real-time tracking of lysosomal oxidative stress and viscosity within living cells

SUBMITTER: Xu Q 

PROVIDER: S-EPMC12848819 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature