Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice.

ABSTRACT:

Introduction

Mental disorders are a severe problem of modern society. Significant in these conditions are the striatal-enriched protein tyrosine phosphatase (STEP) (Ptpn5 gene) and the serotonergic system. Nevertheless, the association between them is poorly studied. The aim of this research was to investigate the effects of Ptpn5 gene knockout on behavior and the serotonin system in mice.

Methods

Utilizing the CRISPR/Cas9 system, we cleaved the PTP-domain-encoding sequence from the Ptpn5 gene of C57BL/6 mice. The resulting strain (Ptpn5 KO) demonstrated STEP protein absence and ERK1/2 hyperphosphorylation (STEP substrate) in the brain. We performed behavioral phenotyping, structural magnetic resonance imaging (MRI) and biomolecular screening of t

SUBMITTER: Moskaliuk V 

PROVIDER: S-EPMC12848919 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature