QuAPPro: an R shiny app for quantification and alignment of polysome profiles.

ABSTRACT:

Background

Polysome profiling is a widespread technique to study mRNA translation. After separation of cellular particles by ultracentrifugation on a sucrose-density gradient, a UV absorbance profile is recorded during elution, which mostly reflects RNA content and shows distinct peaks for ribosomal subunits, monosomes and polysomes with increasing number of ribosomes. This profile can be used to assess global translational activity, or to reveal changes in ribosome biogenesis and translation elongation. In addition, it is also possible to measure the association of fluorescently tagged proteins with ribosomal subunits or polysomes. Alignment and quantification of polysome profiles usually relies on spreadsheet programs, custom R/Python scripts or commercial software.

Results

SUBMITTER: Schiller C 

PROVIDER: S-EPMC12849056 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature