Diagnostic accuracy of ARK Checker® C/G-DyLight® 488: simultaneous detection of Giardia and Cryptosporidium by fluorescent antibody microscopy.

ABSTRACT:

Background

Giardiasis and cryptosporidiosis are often misdiagnosed by stool ova and parasite test (classical O&P). Multiplex PCR and rapid antigen immunochromatography (rapid-IC) could offer high diagnostic accuracy; however, their cost and restricted coverage, especially for parasites, limit routine use. This study evaluated the efficacy of adapted fluorescent antibody microscopy using ARK Checker® C/G-DyLight® 488 (FAM-TEST). The reagent is a liquid-form conjugated antibodies which directly detect Giardia cysts and Cryptosporidium oocysts under fluorescence microscopy.

Methods

A total of 694 stool samples submitted for microbiological examination were screened by FAM-TEST, in which specimens were mixed with DyLight-488-labeled antibodies and examined microscopically. Sampl

SUBMITTER: Oshiro Y 

PROVIDER: S-EPMC12849131 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature