MiR-668-3p in M2 macrophage-derived exosomes activates autophagy through the ETS1/EGFR axis and promotes cisplatin resistance in gastric cancer.

ABSTRACT:

Background

Previous research suggests that tumor-associated macrophages (TAMs) influence the cisplatin (DDP) tolerance of gastric cancer (GC) cells via the secretion of microRNA-containing exosomes. This study aims to investigate the role of exosomal miR-668-3p from M2 macrophages in modulating DDP resistance, using both in vitro and in vivo models to provide a comprehensive analysis.

Materials and methods

The expression profiles of DDP-resistant GC tissues were assessed through microarray, while immunofluorescence confirmed the uptake of these exosomes by GC cells. The role of miR-668-3p in regulating DDP resistance was explored using CCK8 assays, colony formation, EDU incorporation, and Western blotting. The interaction between miR-668-3p and ETS1 was validated through RIP

SUBMITTER: Hu Z 

PROVIDER: S-EPMC12849143 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature